| 1. | Sigourney Weaver, Ghostbusters (1984, 1989), Alien (1979, 1986, 1997), Galaxy Quest (1999) |
| 2. | The Women of Firefly: Gina Torres, Morena Baccarin, Jewel Staite, and Summer Glau in Serenity (2005) |
| 3. | Raquel Welch, Loana in One Million Years B.C. and Cora in The Fantastic Voyage (both in 1966) |
| 4. | Carrie Fisher, Princess Leia Organa in Star Wars (1977) |
| 5. | Halle Berry, Storm/Ororo Munroe |
| 6. | Mathilda May, naked space vampire in Lifeforce (1985) |
| 7. | Milla Jovovich, Leeloo in The 5th Element (1997) |
| 8. | Angelina Jolie, Frankie in Sky Captain and the World of Tomorrow (2004) |
| 9. | Claudia Black, Sharon in Pitch Black (2000) |
| 10. | Ornella Muti, Princess Aura in Flash Gordon (1980) |
| 11. | Jenny Agutter, Jessica in Logan’s Run (1976) |
| 12. | Jane Fonda, Barbarella in Barbarella (1968) |
| 13. | Carrie Anne Moss, Trinity in The Matrix (1999) |
| 14. | Scarlett Johansson, Jordan Two Delta in The Island (2005) |
| 15. | Linda Harrison, Nova in Planet of the Apes (1968) |
| 16. | Natalie Wood, Karen Brace in Brainstorm (1983) |
| 17. | Persis Khambatta, Lt. Ilia in Star Trek the Motion Picture (1979) |
| 18. | Jessica Alba, Sue Storm in The Fantastic Four I and II (2005, 2007) |
| 19. | Megan Fox, Mikaela Banes in Transformers (2007) |
| 20. | Jennifer Connelly, Anna/Emma in Dark City (1998) |
| 21. | Dana Wynter, Becky Driscoll in Invasion of the Body Snatchers (1956) |
| 22. | Mary Elizabeth Mastrantonio, Lindsey Brigman in The Abyss (1989) |
| 23. | Gillian Anderson, Agent Scully in X-Files (1998) |
| 24. | Natalie Portman, Padme Amidalla in Star Wars I-III (1999-2005) |
| 25. | Caroline Munro, Stella Star in Starcrash (1979) |
| 26. | Linda Hamilton, Sara Connor in Terminator 2: Judgment Day (1991) |
| 27. | Rebecca Romijn, Mystique/Grace |
| 28. | Anne Francis, Alraira in The Forbidden Planet (1956) |
| 29. | Cindy Morgan, Lora/Yori in Tron (1982) |
| 30. | Susan Sarandon, Janet in The Rocky Horror Picture Show (1975) |
| 31. | Famke Janssen, Jean Grey |
| 32. | Patricia Neal, Helen Benson in The Day the Earth Stood Still (1951) |
| 33. | Sybil Danning, Saint Exmin in Battle Beyond the Stars (1980) |
| 34. | Dina Meyer, Dizzy Flores in Starship Troopers (1997) |
| 35. | Kim Basinger, Celeste Martin in My Stepmother is an Alien (1988) |
| 36. | Denise Richards, Carmen Ibanez in Starship Troopers (1997) |
| 37. | Faith Domergue, Dr. Ruth Adams in This Island Earth (1955) |
| 38. | Natasha Henstridge, Sil in Species (1995) |
| 39. | Virginia Hey, The Warrior Woman in Mad Maxx 2 (1981) |
| 40. | Daryl Hannah, Pris in Blade Runner (1982) |
| 41. | Madeleine Stowe, Kathryn Railly in 12 Monkeys, (1995) |
| 42. | Kirstie Alley, Saavik in Star Trek II The Wrath of Khan (1982) |
| 43. | Naomi Watts, Jet Girl in Tank Girl (1995) |
| 44. | Elizabeth Peña, voice of Mirage in The Incredibles (2004) |
| 45. | Ally Sheedy, Stephanie in Short Circuit (1987) |
| 46. | Natascha McElhone, Rheya in Solaris (2002) |
| 47. | Kelly LeBrock, Lisa in Weird Science (1985) |