| 1. | Homer Simpson - The Simpsons |
| 2. | Harry Potter |
| 3. | Buffy Summers - Buffy the Vampire Slayer |
| 4. | Tony Soprano - The Sopranos |
| 5. | The Joker - The Dark Knight |
| 6. | Rachel Green - Friends |
| 7. | Edward Scissorhands |
| 8. | Hannibal Lecter - The Silence of the Lambs |
| 9. | Carrie Bradshaw - Sex and the City |
| 10. | Spongebob Squarepants |
| 11. | Cosmo Kramer - Seinfeld |
| 12. | Fox Mulder & Dana Scully - The X-Files |
| 13. | Jack Sparrow - Pirates of the Caribbean |
| 14. | Jeff "The Dude" Lebowski - The Big Lebowski |
| 15. | Shrek |
| 16. | Bridget Jones |
| 17. | Lara Croft |
| 18. | Sue Sylvester - Glee |
| 19. | Morpheus - The Matrix |
| 20. | Ally McBeal |
| 21. | Rosanne Conner |
| 22. | Cartman - South Park |
| 23. | Austin Powers |
| 24. | Felicity Porter |
| 25. | Woody - Toy Story |
| 26. | Kavalier & Clay |
| 27. | Frasier Crane |
| 28. | Madea |
| 29. | Vincent Vega & Jules Winnfield - Pulp Fiction |
| 30. | Stephen Colbert - The Colbert Report |
| 31. | Forrest Gump |
| 32. | Bevis and Butt-Head |
| 33. | Sarah Connor - Terminator 2 |
| 34. | Cher - Clueless |
| 35. | Dexter Morgan - Dexter |
| 36. | Gollum - Lord of the Rings |
| 37. | Kyser Söze - The Usual Suspects |
| 38. | Elmo - Sesame Street |
| 39. | GOB Bluth - Arrested Development |
| 40. | Ron Burgundy - Anchorman |
| 41. | Harold and Kumar |
| 42. | Sydney Bristow - Alias |
| 43. | Cal Stephanides - Middlesex |
| 44. | Jack Bauer - 24 |
| 45. | Stewie Griffin - Family Guy |
| 46. | Jerry Maguire |
| 47. | Corky St. Clair - Waiting for Guffman |
| 48. | Red - The Shawshank Redemption |
| 49. | Vivian Ward - Pretty Woman |
| 50. | Pearl the Landlord - Funny or Die video |
| 51. | Omar Little - The Wire |
| 52. | Annie Wilkes - Misery |
| 53. | Edward Cullen - Twilight |
| 54. | Juno |
| 55. | Tracy Jordan - 30 Rock |
| 56. | Barney Stinson - How I Met Your Mother |
| 57. | Clayton Bigsby (blind white supremacist) - Chappelle's Show |
| 58. | Thelma & Louise |
| 59. | Master Chief - Halo |
| 60. | Mary Jones - Precious |
| 61. | Vic Mackey - The Shield |
| 62. | Jimmy Corrigan - Jimmy Corrigan: The Smartest Kid on Earth |
| 63. | John Locke - Lost |
| 64. | Maximus - Gladiator |
| 65. | Lorelai & Rory Gilmore - Gilmore Girls |
| 66. | Allie & Noah - The Notebook |
| 67. | Borat |
| 68. | (Jennifer Hudson's) Effie White - Dreamgirls |
| 69. | Miranda Priestly - The Devil Wears Prada |
| 70. | Mary Katherine Gallagher - SNL, Superstar |
| 71. | Det. Alonzo Harris - Training Day |
| 72. | Kara "Starbuck" Thrace - Battlestar Galactica |
| 73. | Catherine Tramell - Basic Instinct |
| 74. | Don Draper - Mad Men |
| 75. | David Brent - The Office |
| 76. | Tyler Durden - Fight Club |
| 77. | Mimi Marquez - Rent |
| 78. | Patty Hewes - Damages |
| 79. | Elphaba - Wicked |
| 80. | Gorillaz |
| 81. | Amanda Woodward - Melrose Place |
| 82. | Tracy Flick - Election |
| 83. | Jen Yu - Crouching Tiger, Hidden Dragon |
| 84. | House |
| 85. | Daniel Plainview - There Will Be Blood |
| 86. | Karen Walker & Jack McFarland - Will & Grace |
| 87. | Tony Stark - Iron Man |
| 88. | Napoleon Dynamite |
| 89. | Wilkus van de Merwe - District 9 |
| 90. | Marge Gunderson - Fargo |
| 91. | Hancock (Um, Fresh Prince over this, please) |
| 92. | Christopher Boone - The Curious Incident of the Dog in Night-Time |
| 93. | "Game Boys": Nathan Drake - Uncharted, Kratos - God of War, Niko Bellic - Grand Theft Auto IV |
| 94. | Truman - The Truman Show |
| 95. | Wilhelmina Slater - Ugly Betty |
| 96. | Bernie Mac - The Bernie Mac Show |
| 97. | Violet Weston - August: Osage County |
| 98. | Lisbeth Salander - The Girl with the Dragon Tattoo |
| 99. | The Bride - Kill Bill |
| 100. | Tim Riggins - Friday Night Lights |