| | Original six Gladiators |
| | Malibu (Deron McBee): competed in 1989 only ("The cool, laid-back surfer, at home on the beach") |
| | Lace (Marisa Pare): competed from 1989-1992 ("Feminine, sexy, but always a lady") |
| | Gemini (Michael Horton): competed from 1989-1992 ("A split personality: calm one minute, violent the next") |
| | Zap (Raye Hollitt): competed from 1989-1990, returned from 1991 to 1995 ("Strong, silent, the Terminator") |
| | Nitro (Dan Clark): competed from 1989-1992, returned from 1994-95, color commentator during show's final season ("Cocky, explosive, and always aggressive") |
| | Sunny (Cheryl Barldinger): competed in 1989 only, injured in semifinal round and never returned ("The all-American woman") |
| | Debuted during season one |
| | Blaze (Sha-Ri Pendleton): competed from 1990-1992 |
| | Bronco (Robert Bruce Campbell):competed in one episode in 1989 as an injury replacement for Malibu, was never seen again |
| | Gold (Tonya Knight): competed from 1990-1992 |
| | Laser (Jim Starr): competed from 1990-1996, only Gladiator to appear on all seven seasons of the show |
| | Jade: competed in final episode of first half of season one as injury replacement for Sunny, never seen afterwards |
| | Titan (David Nelson): competed in 1990 only |
| | Debuted during season two |
| | Diamond (Erika Andersch): competed from 1990-1993 |
| | Ice (Lori Fetrick): competed from 1990-1992 and again from 1993-1996 |
| | Thunder (Billy Smith): competed from 1990-1992 |
| | Turbo (Galen Tomlinson): competed from 1990-1996 |
| | Debuted during season three |
| | Storm (Debbie Clark): debuted in 1991 as an injury replacement for Gold, competed until 1993 |
| | Tower (Steve Henneberry): debuted in 1991 as an injury replacement for Turbo, competed until 1994 |
| | Viper (Chuck Berlinger): debuted at 1992 Grand Championship, competed until end of 1992-1993 season |
| | Debuted during season four |
| | Atlas (Philip Poteat): competed in 1992-93 season only |
| | Cyclone (Barry Turner): competed in first half of 1992-93 season, injured arm and didn't return |
| | Elektra (Salina Bartunek): competed from 1992-1994, sparingly after breaking nose in 1993 Grand Championship |
| | Lace #2 (Natalie Lennox): competed in 1992-1993 season only |
| | Havoc: competed sporadically in 1992-93 and 1993-94 seasons |
| | Sabre (Lynn "Red" Williams): competed from 1992-1996 |
| | Siren (Shelley Beattie): competed from 1992-1996, was only deaf Gladiator |
| | Sky (Shirley Eson): competed from 1992-1996, was notable for being a female Gladiator who was over 6 feet tall |
| | Debuted during season five |
| | Dallas (Shannon Hall): competed from second half of this season until 1995, returned with Zap to compete in alumni show in the final season |
| | Hawk (Lee Reherman): competed from 1993-1996 |
| | KevBo (Kevin Gordon): competed from 1993-94 season only |
| | Jazz (Victoria Gay): competed from 1993-1996 |
| | Rebel (Mark Tucker): competed in 1993-94 season only |
| | Debuted during season six |
| | Tank (Ed Radcliffe): competed in three episodes during this season |
| | Thor (Michael O'Hearn): never competed on TV, better known as Michael O'Dell on Battle Dome |